Search results for "nonlinear microscopy"
showing 2 items of 2 documents
Visualization of Keratin with Diffuse Reflectance and Autofluorescence Imaging and Nonlinear Optical Microscopy in a Rare Keratinopathic Ichthyosis.
2021
Keratins are one of the main fluorophores of the skin. Keratinization disorders can lead to alterations in the optical properties of the skin. We set out to investigate a rare form of keratinopathic ichthyosis caused by KRT1 mutation with two different optical imaging methods. We used a newly developed light emitting diode (LED) based device to analyze autofluorescence signal at 405 nm excitation and diffuse reflectance at 526 nm in vivo. Mean autofluorescence intensity of the hyperkeratotic palmar skin was markedly higher in comparison to the healthy control (162.35 vs. 51.14). To further assess the skin status, we examined samples from affected skin areas ex vivo by nonlinear optical micr…
Tunable Four-wave Mixing Light Source Based on Photonic Crystal Fibers with Variable Chromatic Dispersion
2019
We present a detailed experimental study of fourwave mixing tuning in photonic crystal fibers that were filled either with ethanol or with heavy water. It is demonstrated that wide tuning ranges can be achieved in both cases through the variable chromatic dispersion generated by thermo-optic effect. Tunability of the signal band from 745 nm to 919 nm, and of the idler band from 1260 nm to 1759 nm is demonstrated with a pump at 1064 nm. Numerical calculations were carried out and show good agreement with experimental measurements. We present a detailed experimental study of fourwave mixing tuning in photonic crystal fibers that were filled either with ethanol or with heavy water. It is demon…